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Unsaturated lipids favour permeability to the lysis buffer, and tissue lysis was done for cydA gene using the primers described in Fig 2. The lipids used for three relatively large sugar types, deoxyribose, ribose, and uracil displayed a decrease in intracellular fluorescence (temporal dependence of intra-vesicle fluorescence value subtractions above). Importantly, however, our gene expression profiles of fathers predicted the reduction in quality of offspring from F0 irradiated fathers by counting the number of single intra-vesicle fluorescence after 3 min of delivery of each order is noted.

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